All tests were run a minimum of three times to establish some consistency with the results.  No other programs were running in the background, not even a virus scanner. 

First, we have ATTO Disk Bechmark.  Queue Depth is 10, length is 32MB and transfer size is from 0.5 kB to 1024 kB:

As we can see here, write performance slightly exceed read performance until data reached 16k.  At 16k to 1024k, reads average about 43MB/s while writes are at 42MB/s.  Not bad!

The following tests below are of HD Tach v3.0.1.0. 

8MB Zones
Burst:  57.6 MB/s, Ave. Read: 36.2 MB/s, Ave. Write: 27.8 MB/s, CPU: 7%, Random Access: 19.2 mS

32MB Zones
Burst:  91.2 MB/s, Ave. Read: 36.3 MB/s, Ave. Write: 33.6 MB/s, CPU: 6%, Random Access: 17.5 mS

Var Zones
Burst:  91.6 MB/s, Ave. Read: 36.3 MB/s, Ave. Write: 36.1 MB/s, CPU: 3%, Random Access: 16.9 mS

As shown in the screen caps above, the 5400.3 improves its performance as the data size increases.  At the variable zones test, we see 91.6 MB/s in the burst test, a very impressive result.  For the average reads and writes, the data transfer rate is about 36 MB/s in either direction, also very good for a 2.5" HDD.

Because we connected the 5400.3 to the SATA controller, we wanted to see what the controller had to report about the drive:

Lastly, we tried the nForce SATA controller speed test:

As seen here, the results were along the same lines as the other tests above:  82.1 MB/s burst, and 42.8 MB/s sustained.